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acrylamide bis solution  (Bio-Rad)
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native gel containing 6% acrylamide (30(w/v)%-acrylamide/bis mixed solution(37.5:1)  (Nacalai)
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bis-acrylamide solution-30% (37.5:1)  (Hoefer)
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acrylamide/bis-acrylamide solution 37:5:1  (Hoefer)
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acrylamide/bis-acryamide (37.5:1) 40% solution  (Hoefer)
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acrylamide/ bis-acrylamide (37.5-1) solution  (Interchim Chemicals)
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30%(w/v) acrylamide: 0.8% /9w/v) bis-acrylamide stock solution (37.5:1) protogel ec-890  (Fisher Scientific)
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acrylamide/bis-acrylamide (37.5:1) 40% (w/v) solution  (Bio Basic Canada)
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sds−page gels (acrylamide/bis-acrylamide solution, 37.5:1 (30 % w/v), 2.6 % c  (SERVA Electrophoresis)
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acrylamide/bis-acrylamide solution 40% ratio 37.5/1 eu0078-b  (Euromedex)
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KEY RESOURCES TABLE

Journal: Molecular cell

Article Title: Programmable RNA cleavage and recognition by a natural CRISPR-Cas9 system from Neisseria meningitidis

doi: 10.1016/j.molcel.2018.01.025

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Urea Sigma Aldrich #U5378-1KG Trizma base Sigma Aldrich #T1503-5KG Boric acid Sigma Aldrich #B6768-5KG EDTA Sigma Aldrich #EDS-500G DTT Thermo Fisher Scientific #BP17225 HEPES Sigma Aldrich #H4034-100G Proteinase K Thermo Fisher Scientific #FEREO0491 Magnesium chloride hexahydrate Sigma Aldrich #M2670-500G Potassium chloride Sigma Aldrich #P9541-500G Sodium chloride Thermo Fisher Scientific #S271-3 Sodium acetate Sigma Aldrich #S2889-250G Sodium bicarbonate Sigma Aldrich #S5761-500G Heparin Sigma Aldrich #H4784-250MG Glycogen Sigma Aldrich #10901393001 Q5 Hot Start High-Fidelity DNA Polymerase New England Biolabs #M0493L Invitrogen SYBR Safe DNA Gel Stain Thermo Fisher Scientific # {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 Invitrogen Ambion Gel Loading Buffer II Thermo Fisher Scientific #AM8547 Formamide deionized Sigma Aldrich #F9037-100ML Formaldehyde solution Sigma Aldrich #F1635-25ML Orange G Sigma Aldrich #O3756-25G Glycerol Thermo Fisher Scientific #BP229-4 40% Acrylamide/Bis solution, 19:1 Bio-Rad #1610145 30% Acrylamide/Bis solution, 37.5:1 Bio-Rad #1610158 Ammonium persulfate Bio-Rad #A3678-100G TEMED Sigma Aldrich #T9281-50ml Coomassie Brilliant Blue G-250 Amresco #0615-10G LB Broth (Lennox) Sigma Aldrich #L3022-1KG Hydrochloric acid Thermo Fisher Scientific #A14-500 Agar Sigma Aldrich #A1296-1KG IPTG Thermo Fisher Scientific #BP1755-10 FastBreak Cell Lysis Reagent Promega #V8573 Imidazole Sigma Aldrich #I202-500G Bond-Breaker Neutral pH TCEP Solution Thermo Fisher Scientific #PI77720 Tobacco Etch Virus (TEV) protease Ryan Baldridge lab N/A T5 Exonuclease New England Biolabs #M0363S Taq DNA Ligase New England Biolabs #M0208L β-Nicotinamide adenine dinucleotide (NAD+) New England Biolabs #B9007S T4 Polynucleotide Kinase New England Biolabs #M0201S DpnI New England Biolabs #R0176S T4 DNA Ligase New England Biolabs #M0202L Yeast tRNA (10 mg/mL) Thermo Fisher Scientific #AM7119 RNase T1 Thermo Fisher Scientific #EN0541 Critical Commercial Assays AmpliScribe T7-Flash Transcription Kit Epicenter #ASF3507 QIAprep Spin Miniprep Kit QIAGEN #27106 QIAquick Gel Extraction Kit QIAGEN #28706 Ni-NTA agarose QIAGEN #30210 HiTrap Heparin HP GE Healthcare #17-0407-03 HiTrap SP HP GE Healthcare #7115201 HiPrep Sephacryl S-200 HR GE Healthcare #17-1166-01 Experimental Models: Organisms/Strains Escherichia coli JM109 Promega #L2005 Escherichia coli BL21 (DE3) EMD Millipore-Sigma #70235 Oligonucleotides See Table S1 for sequences of oligonucleotides used in this study.

Techniques: Recombinant, Staining, Lysis, Gel Extraction